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【目的】探讨补气开窍法对APP/PS1双转基因小鼠认知能力和β-淀粉样肽42(Aβ42)的影响及其自噬机制。【方法】将56只APP/PS1双转基因小鼠随机分为4组,雌雄各半,每组14只,即模型组、人参总皂苷组(剂量为75 mg/kg)、石菖蒲挥发油组(剂量为15 mg/kg)、补气开窍方组(剂量为石菖蒲挥发油15 mg/kg+人参总皂苷75 mg/kg)。另设正常组,C57BL/6品系的正常小鼠14只,雌雄各半。各组灌胃给药,模型组和正常组用等体积的蒸馏水代替,给药30 d。末次给药结束1 h后,先将各组小鼠进行行为学检测(水迷宫实验,避暗实验和跳台实验),再麻醉各组小鼠,其中4只灌注固定后的全脑用于免疫组织化学法检测Aβ42,其余小鼠在冰上迅速取出海马用于检测Aβ42,流式细胞术检测磷酸化的哺乳动物雷帕霉素蛋白(p-m TOR)和自噬相关基因(Beclin-1)。【结果】与正常组比较,模型组水迷宫实验的潜伏期显著延长(P<0.01),避暗实验和跳台实验的潜伏期显著缩短(P<0.01),Aβ42含量和Beclin-1表达显著增加(P<0.01),p-m TOR表达显著减少(P<0.01);与模型组、人参总皂苷组、石菖蒲挥发油组比较,补气开窍方组的水迷宫实验的潜伏期显著缩短(P<0.05),避暗实验和跳台实验的潜伏期显著延长(P<0.05),水迷宫实验、避暗实验和跳台实验的错误次数显著减少(P<0.05),Aβ42含量显著减少(P<0.05),此外,与模型组比较,补气开窍方组的p-m TOR表达显著增加(P<0.05),Beclin-1表达显著减少(P<0.05)。【结论】人参总皂苷联合石菖蒲挥发油具有改善APP/PS1双转基因模型小鼠的认知能力,减少Aβ42表达和调节自噬作用,且优于单独使用人参总皂苷组或石菖蒲挥发油组。
【Objective】 To investigate the effect of qi-qi-qiao-qiao-qiao on cognitive ability and β-amyloid-42 (Aβ42) in APP / PS1 double transgenic mice and its autophagy mechanism. 【Methods】 Fifty-six APP / PS1 double transgenic mice were randomly divided into 4 groups with 14 male and half female mice. The mice in the model group, the total ginsenoside group (at a dose of 75 mg / kg) Dose of 15 mg / kg), qi Kai resuscitation group (dose of Acorus calamus volatile oil 15 mg / kg + total ginsenoside 75 mg / kg). Another normal group, C57BL / 6 strains of normal mice 14, male and female. Each group was given gavage, and the model group and the normal group were replaced by equal volumes of distilled water for 30 days. At the end of the last administration, behavioral tests (water maze test, avoidance of darkness test and jumping test) were performed on the mice in each group, and then the mice in each group were anesthetized. Four of them were used for immunization Aβ42 was detected by histochemical method, hippocampus was rapidly removed from other mice for detection of Aβ42 on ice, and phosphorylated mammalian target protein (pm TOR) and autophagy-related gene Beclin-1 were detected by flow cytometry. 【Results】 Compared with the normal group, the latent period of water maze test in model group was significantly longer (P <0.01), the latency of avoidance test and jumping test was significantly shorter (P <0.01), the content of Aβ42 and the expression of Beclin-1 (P <0.01). Compared with the model group, the total ginsenoside group and the Acorus gramineus volatile oil group, the latent period of water maze test in QKQ group was significantly shortened (P <0.05) (P <0.05). The numbers of mistakes in water maze test, avoidance test and jumping test were significantly decreased (P <0.05) and the content of Aβ42 was significantly decreased (P <0.05). In addition, compared with the model Compared with the control group, the pm TOR expression was significantly increased (P <0.05), Beclin-1 expression was significantly decreased (P <0.05). 【Conclusion】 Ginseng total saponin combined with Acorus gramineus volatile oil can improve cognitive ability, reduce Aβ42 expression and regulate autophagy in APP / PS1 double transgenic mice, and is better than using ginseng total saponin or Acorus tatarinowii volatile oil alone.