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蜡质芽孢杆菌AR156是南京农业大学与新沂中凯农用化工有限公司合作开发的生物农药。为了解AR156对辣椒的防病促生机制,本文研究了AR156在温室条件下对青枯劳尔氏菌引起的辣椒青枯病的生防效果,对辣椒的促生作用,在辣椒根围的定殖能力,诱导植物细胞防卫反应,如活性氧积累和胼胝质沉积,以及植物防御相关酶的活力。结果表明,蜡质芽孢杆菌AR156对辣椒青枯病的温室防效高达73.31%。AR156的使用使辣椒植株干重增加22.30%,并能稳定的在辣椒根围定殖,接种60 d后,其定殖量为5×105cfu·g-1FW。AR156预处理后挑战接种病原菌能诱导植株更迅速的产生细胞防卫反应,并可显著提高超氧化物歧化酶(SOD)和过氧化物酶(POD)的活力。可见AR156菌株诱导的植物细胞防卫反应,提高植物防御相关酶活和在辣椒根围稳定的定殖能力使其产生对病害的广谱抗性。研究还发现AR156菌剂可增加辣椒叶片叶绿素的含量,这可能是该菌剂促进辣椒生长的原因之一。
The Bacillus cereus AR156 is a biological pesticide developed by Nanjing Agricultural University and Xinyi Zhongkai Agrochemical Co., Ltd. In order to understand the mechanism of disease prevention and growth promoting of AR156 on pepper, this paper studied the biocontrol effect of AR156 on pepper bacterial wilt caused by Ralstonia solanacearum under greenhouse conditions, its promoting effect on pepper, Colonization capacity induces plant cell defense responses such as reactive oxygen accumulation and callose deposition as well as plant defense-related enzyme activity. The results showed that the control effect of Bacillus cereus AR156 on pepper wilt was up to 73.31%. The use of AR156 increased the dry weight of pepper plants by 22.30% and was able to colonize the root of pepper well. After 60 days of inoculation, the colonization rate was 5 × 105cfu · g-1FW. Challenges with AR156 pre-challenge challenge of inoculated pathogens induced more rapid cell defense responses in plants and significantly increased superoxide dismutase (SOD) and peroxidase (POD) activity. It can be seen that the plant cell defense response induced by the AR156 strain enhances the plant defense-related enzyme activity and the stable colonization ability in pepper rhizosphere to produce broad-spectrum resistance to the disease. The study also found that AR156 fungicide can increase the chlorophyll content of pepper leaves, which may be one of the reasons for the fungicide to promote the growth of pepper.