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利用透射电镜详细观察了放线菌酮体内诱导大鼠胸腺细胞凋亡的形态学变化.观察显示,腹腔注射放线菌酮4小时后,大鼠胸腺细胞发生凋亡,凋亡胸腺细胞胞核和胞质发生一系列形态学变化,产生凋亡小体.主要表现为染色质断裂、浓缩、边集,大部分细胞核变成花瓣状,其它细胞核变成半月状、黑洞样和空泡状;粗面内质网大量增殖,并包裹细胞成分形成自噬体;线粒体增多、嵴紊乱并空泡化.凋亡细胞及其形成的凋亡小体被其它细胞吞噬清除.结果表明:放线菌酮体内诱导大鼠胸腺细胞发生自噬性凋亡,凋亡胸腺细胞的细胞器主动参与了凋亡过程.
Transmission electron microscopy (TEM) was used to observe the morphological changes induced by cycloheximide in rat thymocytes induced by actinomycin.The apoptosis of thymocytes in rat thymocytes was observed 4 hours after intraperitoneal injection of cycloheximide, And a series of morphological changes occurred in the cytoplasm, resulting in apoptotic bodies, mainly manifested as chromatin rupture, condensation, edge set, most of the nuclei into petal-like, the other nuclei into a semilunar, black hole-like and vacuolar; The rough endoplasmic reticulum proliferated in a large amount and wrapped the cell components to form autophagosomes. The number of mitochondria increased, the ridges were disorganized and vacuolized. The apoptotic cells and their apoptotic bodies were phagocytosed by other cells. The results showed that actinomycetes Ketone induces autophagic apoptosis in rat thymocytes, and the organelles of apoptotic thymocytes are actively involved in the apoptotic process.