论文部分内容阅读
目的:探讨miR-27a/b对ABCA1及其介导的胆固醇流出的影响。方法:萤光素酶报告基因检测miR-27a/b与ABCA1的结合情况;体外培养THP-1细胞、RAW 264.7细胞和HepG细胞,将miR-27a/b mimics或miR-27a/b inhibitor转染入细
Objective: To investigate the effect of miR-27a / b on ABCA1 and its cholesterol efflux. METHODS: The luciferase reporter gene was used to detect the binding of miR-27a / b to ABCA1. THP-1 cells, RAW 264.7 cells and HepG cells were cultured in vitro and transfected with miR-27a / b mimics or miR-27a / b inhibitor Into fine