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目的探讨子宫颈癌C4-1细胞敲低B-细胞淋巴瘤/白血病-2原癌基因(Bcl-2)的表达,提高人子宫颈癌C4-1细胞对顺铂(DDP)化疗敏感性的作用与机制。方法应用敲低Bcl-2慢病毒转染子宫颈癌C4-1细胞,将其分为对照组、空载组及敲低组,上述分组通过DDP处理后分别作为对照+DDP组、空载+DDP组和敲低+DDP组。采用RT-PCR法检测C4-1细胞Bcl-2 mRNA表达水平,采用流式细胞仪检测C4-1细胞凋亡率,四甲基偶氮唑蓝(MTT)比色法检测C4-1细胞对DDP的敏感性。结果敲低组与空载组及正常对照组比较,C4-1细胞的凋亡率明显增加(P<0.05),C4-1细胞Bcl-2 mRNA表达量降低;敲低组+DDP组与空载+DDP组和对照+DDP组比较IC50值降低,上述差异均有统计学意义(P<0.05)。结论慢病毒转染子宫颈癌C4-1细胞敲低Bcl-2表达可增加凋亡率,提高子宫颈癌C4-1细胞对顺铂的敏感性。
Objective To investigate the expression of Bcl-2 gene knockdown in cervical cancer cell line C4-1 and the sensitivity of human cervical cancer C4-1 cells to cisplatin (DDP) chemotherapy Role and mechanism. Methods The cervical cancer cell line C4-1 was transfected with the Bcl-2 lentivirus. The cells were divided into control group, no-load group and knock-down group. The above groups were treated with DDP as control + DDP group, DDP group and knockdown + DDP group. The expression of Bcl-2 mRNA in C4-1 cells was detected by RT-PCR. The apoptosis rate of C4-1 cells was detected by flow cytometry. The percentage of C4-1 cells was detected by MTT assay DDP sensitivity. Results The apoptosis rate of C4-1 cells was significantly increased (P <0.05), and the expression of Bcl-2 mRNA in C4-1 cells was lower in knockdown group than in control group Compared with the DDP group and the control + DDP group, the IC50 values decreased, the differences were statistically significant (P <0.05). Conclusion The knockdown of Bcl-2 expression by lentiviral transfection of cervical cancer cell line C4-1 can increase the apoptosis rate and enhance the sensitivity of cervical cancer cell line C4-1 to cisplatin.