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目的 :分析2010年江苏省O1群霍乱疫情分离株病原学特征,为霍乱疫情分析及临床治疗提供实验室依据。方法 :对2010年江苏省O1群霍乱疫情分离株进行毒力基因检测、ctx B基因序列分析、抗生素敏感性实验、脉冲场凝胶电泳分子分型分析。结果:2010年江苏省O1群霍乱疫情分离株均携带毒力基因ctx A、ace、zot、tox R、tcp I、omp U、rtx C、tcp AEL、hly AEL,且CTXB氨基酸序列为古典型;对庆大霉素、诺氟沙星、环丙沙星100%敏感,对复方新诺明、链霉素100%耐药,且均为多重耐药株;除VC201014外,其余14株菌脉冲场凝胶电泳图谱相似度达100%。结论:2010年9月初至10月初,发生在江苏省徐州、淮安、宿迁、南京4个地市的O1群霍乱疫情可能是有关联的暴发流行,2010年8月底发生在连云港的O1群霍乱疫情是一起散发疫情,与上述四地的霍乱疫情可能没有流行病学关联,所有疫情的病原均为非典型埃尔托型霍乱弧菌,庆大霉素、诺氟沙星、环丙沙星可作为临床治疗的首选药物。
OBJECTIVE: To analyze the etiological characteristics of the cholera outbreak isolates from the O1 population in Jiangsu Province in 2010 and provide a laboratory basis for the analysis and clinical treatment of the cholera epidemic. Methods: The virulence genes, ctx B gene sequence analysis, antibiotic sensitivity test and pulsed-field gel electrophoresis molecular typing were analyzed in 2010 isolates of cholera epidemic in Jiangsu province. Results: The isolates of cholera in O1 group in Jiangsu Province all carried the virulence genes ctx A, ace, zot, tox R, tcp I, omp U, rtx C, tcp AEL and hly AEL in 2010 and the amino acid sequence of CTXB was classical. For gentamicin, norfloxacin, ciprofloxacin 100% sensitive to compound cotinine, streptomycin 100% resistant, and are multi-resistant strains; in addition to VC201014, the remaining 14 strains of bacteria pulse Field gel electrophoresis pattern similarity of 100%. Conclusion: From the beginning of September to the beginning of October 2010, the cholera outbreak in O1 group in 4 cities of Xuzhou, Huaian, Suqian and Nanjing in Jiangsu Province may be associated with outbreak. In the end of August 2010, the cholera outbreak in O1 group occurred in Lianyungang Together with the above-mentioned four epidemics of cholera, there may be no epidemiological correlation. The pathogens of all outbreaks are atypical El Tor-type V. cholerae, gentamycin, norfloxacin and ciprofloxacin As the preferred drug for clinical treatment.