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本文研究了亚硒酸钠(sodium selenice,Se)和1,6-二磷酸果糖(fructose-1,6-diphosphate,FDP)对乳鼠培养心肌细胞缺血早期影响的形态计量学和定量细胞化学。用图像分析系统(textureanalysis system,TAS)同步动态定量测定了心肌细胞和亚细胞的形态以及细胞化学的改变,并观察了心肌细胞脂质过氧化(lipid peroxidation,LPO)和搏动功能的改变。结果提示:线粒体体积和数目的改变呈高度负相关,当线粒体肿胀超过其自身大小的一倍时,溶解破坏增多;Se和FDP对缺血心肌细胞均有保护作用,但Se对防止线粒体溶解,维持心肌细胞的琥珀酸脱氢酶(succin c dehydrogenase,SDH)活性及降低缺血心肌细胞丙二醛(malondialodehyde,MDA)含量等作用明显强于FDP,而FDP减轻心肌细胞和线粒体肿胀,改善心肌细胞搏动的作用强于Se。
In this paper, we studied the early morphological and quantitative cytochemical effects of sodium selenice (Se) and fructose-1,6-diphosphate (FDP) on neonatal rat cardiomyocytes ischemia . The morphological and cytochemical changes of cardiomyocytes and sub-cells were synchronously and dynamically determined by the image analysis system (TAS). Changes in cardiomyocyte lipid peroxidation (LPO) and pulsatile function were also observed. The results suggest that the mitochondrial volume and number of changes were highly negatively correlated, when the mitochondrial swelling more than double its size, the dissolution damage increased; Se and FDP on ischemic cardiomyocytes have a protective effect, but Se to prevent mitochondrial dissolution, The effect of maintaining succinate dehydrogenase (SDH) activity and reducing the content of malondialodehyde (MDA) in myocardial cells was significantly stronger than that of FDP, while FDP could reduce the swelling of myocardial cells and mitochondria, improve myocardial function Cell beating is stronger than Se.