采用高效液相色谱法，以电化学检测测定双黄连粉针中黄芩甙和黄芩素的含量。流动相０１ｍｏｌ／Ｌ磷酸二氢钾乙酸四氢呋喃（１０００∶１００∶１９０），色谱柱ＥＲＣＯＤＳ１１６１（６ｍｍ×１００ｍｍ），检测电压＋６５０ｍＶ，柱温５５℃，流速０８ｍｌ／ｍｉｎ。结果：本法的最小检测限为２５ｎｇ和５０ｎｇ，平均回收率为１００１％和９９７％，重现性好。本法测定快速、简便、准确，适用于含黄芩甙和黄芩素的中药材和制剂的含量测定。 The content of Scutellaria baicalensis and Scutellarin in Shuanghuanglian powder injection was determined by electrochemical detection with high performance liquid chromatography. Mobile phase: 01mol/L potassium dihydrogen phosphateacetic acidtetrahydrofuran (1000∶100:190), column ERCODS1161 (6mm×100mm), detection voltage +650mV, column temperature 55°C, flow rate 08ml/ Min. Results: The minimum detection limit of this method was 25 ng and 50 ng, and the average recovery was 100  1% and 99  7%. The reproducibility was good. The method is rapid, simple and accurate. It is applicable to the determination of Chinese herbal medicines and preparations containing Astragalus membranaceus and baicalein.