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采用形态学和EF-1α基因序列分析相结合的方法,对我国13个省17个市区的274份高粱子粒样品中分离获得的313株镰孢菌进行鉴定。在313株镰孢菌中,共鉴定出13个镰孢菌种:产黄色镰孢(Fusarium thapsinum)、禾谷镰孢(F.graminearum)、层出镰孢(F.proliferatum)、变红镰孢(F.incarnatum)、藤仓镰孢(F.fujikuroi)、轮枝镰孢(F.verticillioides)、亚粘团镰孢(F.subglutinans)、木贼镰孢(F.equiseti)、茄镰孢(F.solani)、尖孢镰孢(F.oxysporum)、九州镰孢(F.kyushuense),尚无中文名称的F.miscanthi和F.lacertarum。产黄色镰孢(F.thapsinum)、禾谷镰孢(F.graminearum)、层出镰孢(F.proliferatum)和变红镰孢(F.incarnatum)是高粱子粒寄藏镰孢菌种群中的优势种类。从分离频率和地域分布广泛性来看,我国高粱产区高粱子粒上均可分离获得镰孢菌,河南、山东、四川的分离频率最高,分别达43.87%、43.73%和40.44%。
A total of 313 Fusarium isolates from 274 sorghum seeds isolated from 17 urban areas in 13 provinces of China were identified by morphological and EF-1α gene sequence analysis. Of 313 Fusarium strains, 13 Fusarium species were identified: Fusarium thapsinum, F.graminearum, F.proliferatum, F. incarnatum, F. fujikuroi, F. verticillioides, F. subglutinans, F. equiseti, F. solani, F.oxysporum, F. yushuense, F. miscanthi and F. lacertarum without Chinese name. Production of F.thapsinum, F.graminearum, F.proliferatum, and F.incarnatum are among the populations of sorghum fusarium stock fusarium The type of advantage. In terms of separation frequency and geographical distribution, Fusarium was isolated from sorghum producing sorghum in China, and the highest frequency was 43.87%, 43.73% and 40.44% respectively in Henan, Shandong and Sichuan.