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Pathak等曾发现具有不同二倍体的8个物种的纤维母细胞的姊妹染色单体互换(SCE)率很相似,而kato报告23个物种的培养细胞的SCE率与染色体组型有关,但这两份报告都只用了一个剂量的5-澳脱氧尿核苷(BrdUrd)。本文报告了不同剂量的BrdUrd对人和牛、羊、猪的SCE率的影响。实验用健康人四名和牛、羊、猪各四头,采集淋巴细胞,其浓度均调至10~4/mm~3,分别加1 ml于含0.5、1、2、5、10和20μg BrdUrd的10 ml的完全培养基中,并把淋巴细胞用植物凝血素(PHA)(人、羊和猪)和商陆籽
Pathak et al. Found that the sister chromatid exchange (SCE) rates of fibroblasts of eight species with different diploids are very similar, whereas Kato reports that the SCE rates of cultured cells from 23 species are related to the karyotype but Both reports use only one dose of 5- dUdr. This article reports the effects of different doses of BrdUrd on the SCE rates in humans and cattle, sheep and pigs. Four healthy subjects and four heads of cattle, sheep and pigs were collected. The lymphocytes were collected and the concentration of them was adjusted to 10 ~ 4 / mm ~ 3. The animals were treated with 1 ml each of 0.5, 1, 2, 5, 10 and 20 μg BrdUrd Of 10 ml of complete medium, and the lymphocytes were labeled with phytohemagglutinin (PHA) (human, sheep and pig) and pokeberry seeds