论文部分内容阅读
将优质抗稻瘟病水稻恢复系成恢177与转基因水稻Bt明恢63杂交并回交1次,采用PCR分析、试纸条检测、室内和田间人工接虫鉴定抗虫性,并结合系谱法选择农艺性状,病圃接种鉴定稻瘟病抗性,育成具转基因抗虫性的水稻新恢复系Bt5198。采用离体茎秆法接种二化螟卵块,新恢复系Bt5198和Bt明恢63的幼虫死亡率均为100%。在田间人工接虫条件下,该恢复系对二化螟和三化螟均表现高抗,与4个不育系配制的杂种F1仍保持良好的抗虫性,且杂种优势明显。两年稻瘟病抗性鉴定结果表明,新恢复系Bt5198的叶瘟和颈瘟抗性水平与成恢177相当,明显优于Bt明恢63。Bt5198的种子发芽率和花粉量与成恢177相当,Bt基因导入水稻恢复系不会对种子生活力和制种产量造成显著影响。在无选择标记基因的转基因后代中,结合利用试纸条检测和室内、田间人工接虫鉴定是筛选Bt基因抗虫性的有效方法。
High-quality rice blast restorer Chenghui177 and transgenic rice Bt Minghui63 were crossed and backcrossed for 1 time. PCR analysis, test strip test, indoor and field pest management were used to identify pest resistance, Agronomic traits, inoculation of disease nursery stock to identify blast resistance, and breeding of new restorer line Bt5198 with transgenic pest resistance. Inoculation of the rice stem borer egg masses by in vitro stalk larvae mortality were 100% for the new restorer lines Bt5198 and Bt Minghui63. The restorer lines showed high resistance to both the rice stem borer and the rice stem borer (Borer) and the mixed F1 hybrids with the four sterile lines maintained good insect resistance and obvious heterosis under field conditions. Two years of blast resistance identification results showed that the new restorer line Bt5198 leaf blast and neck blast resistance levels and Cheng Hui 177 equivalent, significantly better than the Bt Ming Hui 63. The seed germination rate and pollen amount of Bt5198 were equivalent to that of Chenghui 177, and the introduction of Bt gene into rice restorer lines would not significantly affect seed viability and seed yield. In transgenic progenies without selectable marker genes, the combination of test strip test and laboratory and field identification of artificial insects was an effective method to screen Bt insect resistance.