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目的:考察番茄红素的抗大鼠脑缺血活性,探讨其线粒体作用机制。方法:100只雄性Wistar大鼠随机分为假手术组、模型组、番茄红素低、中、高剂量组(50,100和200 mg.kg-1),各组均在缺血前1 h灌胃给药。采用Longa线栓法造成大鼠大脑中动脉阻断后再灌注模型,缺血2 h,再灌注24 h,观察大鼠神经行为学表现、脑组织梗死率以及脑组织水肿程度的改变;同时检测缺血侧(损侧)脑组织ATP含量、线粒体膜电位水平及线粒体呼吸链复合酶IV活性。结果:番茄红素能明显改善脑缺血再灌注引起的神经行为学评分降低,显著降低脑组织梗死率和水肿程度。番茄红素能增加缺血脑组织的ATP含量,提高线粒体膜电位水平,增强呼吸链复合酶IV活性。结论:番茄红素对大鼠的脑缺血再灌注损伤有明显保护作用,其机制可能与保护和改善细胞能量代谢和线粒体功能有关。
OBJECTIVE: To investigate the anti-cerebral ischemia activity of lycopene and its mitochondrial mechanism of action. Methods: 100 male Wistar rats were randomly divided into sham operation group, model group, low, medium and high dose lycopene group (50, 100 and 200 mg.kg-1) Administration. The rat model of middle cerebral artery occlusion (MCAO) was established by Longa suture method. The rats were subjected to ischemia reperfusion 2 h after ischemia and reperfusion for 24 h. The changes of neurobehavioral performance, infarct rate and brain edema were observed. ATP content, mitochondrial membrane potential and mitochondrial respiratory chain enzyme IV activity in ischemic (lesioned) brain tissue. Results: Lycopene could obviously reduce the score of neurobehavioral injury caused by cerebral ischemia and reperfusion, and significantly reduce the rate of cerebral infarction and the degree of edema. Lycopene can increase the content of ATP in ischemic brain tissue, increase the level of mitochondrial membrane potential and enhance the activity of respiratory chain complex enzyme IV. CONCLUSION: Lycopene has a significant protective effect on cerebral ischemia-reperfusion injury in rats and its mechanism may be related to the protection and improvement of cell energy metabolism and mitochondrial function.