论文部分内容阅读
目的探讨真核延伸因子1A1(eEF1A1)在T1N0M0期肾癌组织中的表达及其与预后的关系。方法收集第二军医大学长海医院泌尿外科在2007年行手术治疗的患者标本共70例。入选标准:术前未进行其他治疗,TNM分期为T1N0M0,行肾癌根治术,病理为透明细胞癌,患者及随访资料完整。通过免疫组化技术检测手术标本中癌组织eEF1A1的表达量,建立eEF1A1表达量与Fuhrman病理分级和预后之间的对应关系,绘制交叉表,通过χ2检验、Fisher精确概率法研究eEF1A1和肾癌Fuhrman病理分级的关系。通过绘制Kaplan-Meier生存曲线并进行log-rank检验研究eEF1A1和T1N0M0期肾癌预后的关系。结果随访时间为4~95个月,平均(77.9±18.3)个月,随访期内总生存率为88.6%,无进展生存率为80.0%。免疫组化显示eEF1A1在61.4%的肾癌组织中高表达,并且在高Fuhrman分级的肾癌组织中高表达(P=0.004)。生存分析提示肾癌组织低表达eEF1A1的患者生存期较长(P=0.019),并且癌组织中低表达eEF1A1的患者均存活。结论 T1N0M0期肾癌组织中eEF1A1表达与肿瘤Fuhrman病理分级及患者预后有关,表达量低的患者预后较好。
Objective To investigate the expression of eEF1A1 in T1N0M0 stage renal cell carcinoma and its relationship with prognosis. Methods A total of 70 patients undergoing surgery in Department of Urology, Changhai Hospital, Second Military Medical University in 2007 were collected. Inclusion criteria: no other preoperative treatment, TNM staging T1N0M0, radical nephrectomy, the pathology of clear cell carcinoma, patients and follow-up data integrity. The expression of eEF1A1 in cancer tissue was detected by immunohistochemistry and the relationship between eEF1A1 expression and Fuhrman pathological grade and prognosis was established. Crosstabs were drawn and analyzed by χ2 test and Fisher’s exact test for eEF1A1 and Fuhrman Pathological classification of the relationship. Kaplan-Meier survival curves were plotted and log-rank test was performed to investigate the relationship between eEF1A1 and T1N0M0 stage renal cell carcinoma prognosis. Results The follow-up time ranged from 4 to 95 months with an average of (77.9 ± 18.3) months. The overall survival rate was 88.6% at follow-up, and the progression-free survival rate was 80.0%. Immunohistochemistry showed that eEF1A1 was overexpressed in 61.4% of renal cancer tissues and highly expressed in high Fuhrman grade renal cell carcinoma (P = 0.004). Survival analysis suggested that patients with low expression of eEF1A1 in renal cell carcinoma had longer survival (P = 0.019), and patients with low expression of eEF1A1 in cancer tissues survived. Conclusion The expression of eEF1A1 in T1N0M0 stage renal carcinoma is related to the tumor Fuhrman pathological grading and prognosis. The patients with low expression level have a better prognosis.