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目的:观察滨蒿总黄酮提取物(滨蒿总黄酮)在体内外的抗流感病毒作用。方法:体外抗病毒实验观察样品对狗肾传代细胞(MDCK)的毒性,观察病毒致细胞病变(CPE)法测定样品在体外抑制甲、乙型流感病毒的半数抑制浓度(IC50),并计算选择指数(SI)。体内抗病毒实验用流感病毒鼠肺适应株(PR8)滴鼻感染小鼠建立流感病毒性肺炎模型,以死亡保护率、生命延长率和肺指数等为指标,评价样品的保护作用。结果:滨蒿总黄酮对甲、乙型流感病毒致细胞病变的IC50分别为74.6μg/mL、98.5μg/mL,SI分别为16.8、12.7;滨蒿总黄酮以200、400mg/kg剂量灌胃给药对小鼠流感病毒性肺炎模型有明显的保护作用,其死亡保护率分别为36.4%、54.5%;生命延长率分别为29.0%、38.2%;滨蒿总黄酮400mg/kg剂量对流感病毒性肺炎小鼠肺指数有明显的抑制作用,抑制率为25.9%。结论:滨蒿总黄酮在体内外均有明显的抗病毒作用。
Objective: To observe the anti-influenza virus effect of total flavonoids from Artemisia selengensis (Artemisia cirrhosa) in vivo and in vitro. Methods: The in vitro anti-virus test was used to observe the toxicity of the sample to the MDCK. The inhibitory concentration (IC50) of the samples was determined in vitro by the CPE method. Index (SI). In Vivo Antiviral Experiment Influenza virus pneumonia models were established by intranasal infection of mice with influenza virus murine lung adaptive strain (PR8). The protective effect of the samples was evaluated using the death protection rate, the life prolongation rate and the lung index as indices. Results: The IC50 of total flavonoids of Artemisia selengensis was 74.6μg / mL and 98.5μg / mL for influenza A and B viruses respectively, SI was 16.8 and 12.7, respectively. The total flavonoids of Artemisia selengensis were orally administered at doses of 200 and 400mg / kg The protective rate of death was 36.4% and 54.5%, respectively. The life prolongation rates were 29.0% and 38.2%, respectively. The dose of 400 mg / kg of total flavonoids of Artemisia selengensis was significantly lower than that of influenza virus Pneumonia mouse lung index was significantly inhibited, the inhibition rate was 25.9%. Conclusion: Artemisia selengensis total flavonoids have obvious antiviral effect both in vitro and in vivo.