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磷脂酶D(Phospholipase D,PLD)是在植物组织中广泛存在的一类磷脂酶,可催化磷脂如磷脂酰胆碱(phosphatidylcholine)水解产生磷脂酸(phosphatidic acid,PA)和一个自由的头部基团如胆碱(choline)。在植物体内PLD家族往往包括多个成员。利用反向遗传学技术对水稻磷脂酶D家族(OsPLD)中的两个成员OsPLD3和OsPLD4基因及其启动子的研究显示:OsPLD3和OsPLD4的启动子在花器官的不同部位中驱动报告基因不同程度地表达,二者都受损伤和茉莉酸甲酯诱导,但是对诱导因子反应的时空模式不同。利用转基因技术在水稻中过量表达OsPLD3和OsPLD4基因或是干扰OsPLD3和OsPLD4基因表达都不能引起可见的水稻表型的变化,说明OsPLD家族不同成员可能有功能上的重复。
Phospholipase D (PLD) is a type of phospholipase widely found in plant tissues. It catalyzes the hydrolysis of phospholipids such as phosphatidylcholine to produce phosphatidic acid (PA) and a free head group Mission such as choline. PLD families in plants often include multiple members. The reverse genetics of OsPLD3 and OsPLD4 genes and their promoters in two members of the rice phospholipase D family (OsPLD) revealed that the promoters of OsPLD3 and OsPLD4 drive different levels of reporter genes in different parts of floral organs Both were impaired and induced by methyl jasmonate, but had different spatiotemporal patterns of induction response. Overexpression of OsPLD3 and OsPLD4 genes in rice or interference with the expression of OsPLD3 and OsPLD4 in transgenic rice did not result in any visible phenotypic changes in rice, suggesting that functional duplication may exist between different members of the OsPLD family.