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建立了HPLC-DAD法检查中药制剂违禁添加的9种化学止咳平喘药——富马酸酮替芬、硫酸特布他林、茶碱、乙羟茶碱、咖啡因、盐酸克伦特罗、愈创甘油醚、氢溴酸右美沙芬和盐酸氨溴索。采用C18色谱柱,以0.75%磷酸-甲醇为流动相,梯度洗脱,检测波长254 nm。富马酸酮替芬和愈创甘油醚在0.6～6μg、其它7种药物在0.2～2μg范围内线性关系良好,回收率为98.3%～99.6%,RSD均小于1.2%。
A HPLC-DAD method was established for the determination of nine chemical anti-cough and antiasthmatic drugs prohibited by traditional Chinese medicine preparations - ketotifen fumarate, terbutaline sulfate, theophylline, acetophenone, caffeine, clenbuterol , Guaifenesin, dextromethorphan hydrobromide and ambroxol hydrochloride. C18 column with 0.75% phosphoric acid - methanol as mobile phase, gradient elution, detection wavelength 254 nm. The ketotifen fumarate and guaifenesin were in the range of 0.6-6μg. The other seven drugs showed a good linearity in the range of 0.2-2μg. The recoveries were 98.3% -99.6% with RSD less than 1.2%.