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研究钙内流对原代培养的皮质和海马神经元内ｐＨ（ｐＨｉ）的影响。为了达到此目的，暴露单个神经元于谷氨酸（Ｇｌｕ，１００μｍｏｌ／Ｌ）或氯化钾（ＫＣｌ，５０ｍｍｏｌ／Ｌ）。用微荧光法测定细胞内游离钙（〔Ｃａ２＋〕ｉ）和ｐＨｉ。结果表明，Ｇｌｕ暴露或Ｋ＋－诱导的去极化可导致海马神经元明显酸化（△ｐＨ～０．４）。在无钙溶液中，这一ｐＨ降低被显著减少（Ｇｌｕ暴露）或转化成增高（Ｋ＋－诱导的去极化）。相反，皮质神经元对Ｇｌｕ的反应很弱，只有轻微的酸化。实际上，去极化诱导的ｐＨｉ在两种细胞中呈反方向变化。由上述结果得出结论：海马与皮质神经元的不同是海马神经元在钙通道激活后允许更大量的钙离子从细胞外液流入。
To study the effects of calcium influx on the primary culture of cortical and hippocampal neurons pH (pHi). To do this, single neurons were exposed to glutamate (Glu, 100 μmol / L) or potassium chloride (KCl, 50 mmol / L). Intracellular free calcium ([Ca2 +] i) and pHi were determined by the micro-fluorescence method. The results show that Glu exposure or K + -induced depolarization can lead to significant acidification of hippocampal neurons (△ pH ~ 0.4). In calcium-free solutions, this decrease in pH was significantly reduced (Glu exposure) or converted to increase (K + -induced depolarization). In contrast, cortical neurons reacted poorly to Glu with only slight acidification. In fact, the depolarization induced pHi varied in the opposite direction in both cells. From the above results, it is concluded that the difference between the hippocampus and the cortical neurons is that hippocampal neurons allow a greater amount of calcium ions to flow from the extracellular fluid upon activation of the calcium channel.