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目的:探讨金雀异黄素对体外高糖条件下大鼠系膜细胞(MC)增殖及凋亡的影响.方法:对照组:低糖DMEM培养液(不含金雀异黄素),实验组:高糖DMEM培养液(含0,1,5,10,30,50μmol/L金雀异黄素),各组分别培养12,24,36,48 h.氮蓝四唑盐(MTT)法检测MC的增殖情况,DNA琼脂糖电泳、末端转移酶介导的dUTP缺口末端标记(TUNEL)法、流式细胞术3种方法检测细胞凋亡情况.结果:10,30,50μmol/L金雀异黄素作用24 h可明显诱导细胞凋亡(P<0.05),而0,1,5μmol/L作用不显著.随着金雀异黄素浓度的增加,凋亡比例增加;同一浓度的金雀异黄素随着作用时间的延长,凋亡比例亦增加.结论:一定浓度的金雀异黄素在体外高糖培养条件下,可抑制大鼠MC增殖,促进其凋亡,且与其浓度和作用时间有关.
Objective: To investigate the effect of genistein on the proliferation and apoptosis of rat mesangial cells (MC) under high glucose conditions in vitro. Methods: Control group: low glucose DMEM medium (without genistein), experimental group : High-glucose DMEM medium (containing 0, 1, 5, 10, 30, 50 μmol/L of genistein), cultured in each group for 12, 24, 36, and 48 h. The nitroblue tetrazolium salt (MTT) method The proliferation of MC was detected, DNA agarose electrophoresis, terminal transferase-mediated dUTP nick end labeling (TUNEL), and flow cytometry were used to detect apoptosis. Results: 10, 30, 50 μmol/L. After 24 h of isoxanthin treatment, apoptosis was significantly induced (P<0.05), but 0, 1 and 5 μmol/L had no significant effect. With the increase of genistein concentration, the proportion of apoptosis increased; the same concentration of gold increased. With the prolongation of time, the percentage of apoptotic cells increased with the increase of time. Conclusion: The concentration of genistein in a certain concentration of high glucose can inhibit the proliferation of rat MC, promote its apoptosis, and its concentration and role Time related.