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本文采用 SD成年大白鼠饮用醋酸铅水染毒 30天 ,然后用改进的 NADPH测定法及免疫组化 SP法观察脑组织中 NOS酶活性及 NOS酶蛋白亚型的变化。大白鼠分为五组 ,3个剂量组 ,高铅组为 2 0 .0 g/ L 醋酸铅 ,中铅组为 5 .0 g/ L 醋酸铅 ,低剂量为 1.0 g/ L 醋酸铅 ,分别经饮水染毒 ;阳性对照组为 1.0 g/ L 天冬酸 ;阴性对照组为普通自来水。结果显示 ,染铅组脑组织海马及小脑的 NOS酶活性水平明显下降 ,小脑区 n NOS及 i NOS蛋白与对照相比无显著性差异 ,而海马区的i NOS酶蛋白的表达高于对照组。这一结果表明 ,铅染毒后对大鼠中枢神经系统中 NOS酶活性具有抑制作用 ,这可能是铅中毒引起学习记忆功能障碍的重要机制之一。
In this paper, SD rats were challenged with lead acetate drinking water for 30 days. Then the NOS enzyme activity and NOS enzyme subtypes in brain were observed by modified NADPH assay and immunohistochemical SP method. Rats were divided into five groups and three dose groups. The lead group was 20.0 g / L lead acetate, the lead group was 5.0 g / L lead acetate and the low dose was 1.0 g / L lead acetate. Toxic; positive control group was 1.0 g / L aspartic acid; negative control group of ordinary tap water. The results showed that the levels of NOS enzyme in the hippocampus and cerebellum of the lead-exposed group were significantly decreased. There were no significant differences in nNOS and iNOS protein in the cerebellar region compared with the control group, while the expression of iNOS enzyme in hippocampus was higher than that in the control group . This result indicates that lead poisoning can inhibit the activity of NOS in the central nervous system of rats, which may be one of the important mechanisms of lead poisoning in learning and memory dysfunction.