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目的:建立芍药苷醇质体含量及包封率的测定方法。方法:采用注入法制备醇质体,以超速离心法分离醇质体和游离药物,建立HPLC测定芍药苷含量的方法。结果:芍药苷浓度在0.966~4.8μg范围内与峰面积呈良好的线性关系(r=0.9997),回收率为97.6%~100.8%,日内及日间精密度均小于1.5%,样品8 h内稳定性良好,平均包封率为81.03%。结论:该方法准确可靠方便快捷,可用于芍药苷醇质体中药物含量及包封率的测定。
Objective: To establish a method for the determination of paeoniflorin and encapsulation efficiency. Methods: Ethosomes were prepared by injection method, and the apoliposomes were separated by ultracentrifugation. The HPLC method was established for the determination of paeoniflorin. Results: Paeoniflorin had a good linear relationship with the peak area in the range of 0.966-4.8μg (r = 0.9997) and the recovery was 97.6% -100.8%. The intra-and inter-day precision was less than 1.5% Good stability, the average entrapment efficiency was 81.03%. Conclusion: The method is accurate and reliable, convenient and fast, and can be used for the determination of drug content and entrapment efficiency of paeoniflorin.