本文叙述了我国产蓖麻(Ricinus communis L)种子的含毒提取液在CM-纤维素(CM32)柱层析时用含0.1mol/L NaCl、0.05mol/L的磷酸缓冲液(pH6.5)洗脱,分出3个有毒馏份,再经Sepharose 4B和(或)Sephadex G-100柱层析得到3种蓖麻毒素和1种蓖麻凝集素。通过SDS-聚丙烯酰胺凝胶电泳测定,蓖麻凝集素的分子量是125 000 Da,蓖麻毒素a、b和c的分子量分别63 000、63 000和56000Da,对小鼠腹腔注射LD_(50)为11.9、79.0和12.3μg/kg。蓖麻凝集素在0.03μg/ml,4℃放置4h This paper describes the toxic extracts of Ricinus communis L seeds in China using CM-cellulose (CM32) column chromatography with 0.1mol / L NaCl, 0.05mol / L phosphate buffer (pH6.5 ), Three toxic fractions were separated, and three kinds of ricin and one type of castor bean lectin were obtained by column chromatography on Sepharose 4B and / or Sephadex G-100. The molecular weight of ricin agglutinin was 125 000 Da and the molecular weights of ricin a, b and c were 63 000, 63 000 and 56 000 Da, respectively. The intraperitoneal injections of LD 50 were performed by SDS-polyacrylamide gel electrophoresis. 11.9, 79.0 and 12.3 μg / kg. Castor Lectin was placed at 4 ° C for 4 h at 0.03 μg / ml