论文部分内容阅读
为了进一步验证骨膜成骨细胞培养建立“成骨细胞库”用于修复骨缺损的可能性，取８只家兔胫前骨膜进行成骨细胞分离培养，体外繁殖后，以明胶海绵为载体，回植于自体的桡骨缺损部。对侧以明胶海绵吸附Ｈａｎｋｓ液为对照。分别在细胞植入后第２，４，８和１２周拍摄Ｘ线片，按Ｌａｎｅ方法计分，评价其骨缺损修复愈合的能力。结果表明，植入的成骨细胞因自身的成骨能力而促进骨缺损的愈合。提示自体“成骨细胞库”的建立和移植在骨缺损的修复中是一种可以继续探索的方法
In order to further verify the possibility of establishing “osteoblast reservoir” for repairing bone defects in periosteal osteoblast culture, 8 rabbits were sacrificed and the osteoblasts were isolated and cultured in vitro. After in vitro culture, gelatin sponge was used as the carrier, Implanted in the body of the radial defect. Contralateral to gelatin sponge adsorption Hankss solution as a control. X-ray films were taken at the 2nd, 4th, 8th and 12th weeks after cell implantation, respectively, and were scored according to the Lane method to evaluate the ability of bone defect repair healing. The results show that the implanted osteoblasts promote the healing of bone defects due to their osteogenic ability. It is suggested that the establishment and transplantation of autologous “osteoblast repositories” is a method that can be explored in the repair of bone defects