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目的探讨CD166对鼻咽癌CNE-2细胞放射敏感性的影响。方法采用流式细胞术检测鼻咽癌CNE-2细胞膜CD166阳性表达率,免疫磁珠分选技术获得CNE-2-CD166(+)、CNE-2-CD166(-)细胞亚群,克隆形成实验验证其放射敏感性,CCK-8法及流式细胞术检测10 Gy剂量照射前后的细胞增殖率及细胞凋亡率。结果 CNE-2细胞膜CD166阳性表达率为(24.27±5.31)%,分选后CNE-2-CD166(+)细胞CD166阳性表达率为96.5%,CNE-2-CD166(-)细胞为0.6%。克隆形成实验结果显示,CNE-2-CD166(-)细胞相对CNE-2-CD166(+)细胞的放射增敏比(sensitization enhancement ratio,SER)为1.24。10 Gy照射后24 h、48 h和72 h,CNE-2-CD166(+)细胞的存活分数分别为(76.66±3.36)%、(62.44±1.66)%和(55.21±3.39)%,CNE-2-CD166(-)细胞为(60.34±5.13)%、(41.11±3.00)%和(35.28±4.36)%,同时段内两组细胞的存活分数比较,差异均有统计学意义(P<0.05)。10 Gy照射前,CNE-2-CD166(+)细胞和CNE-2-CD166(-)细胞凋亡率分别为(5.87±1.14)%和(6.67±1.68)%,差异无统计学意义(P>0.05);10 Gy照射后48 h,CNE-2-CD166(+)细胞凋亡率为(19.37±2.54)%,显著低于CNE-2-CD166(-)细胞的(28.90±4.04)%,差异有统计学意义(P=0.026)。结论 CD166表达阳性的鼻咽癌CNE-2细胞对放射更具抗拒性,可能与减少细胞受照射后的凋亡率降低有关。
Objective To investigate the effect of CD166 on the radiosensitivity of nasopharyngeal carcinoma CNE-2 cells. Methods The positive rate of CD166 in CNE-2 cell line was detected by flow cytometry. The CNE-2-CD166 (+) and CNE-2-CD166 The radiosensitivity of the cells was examined by CCK-8 assay and flow cytometry before and after irradiation with 10 Gy. Results The positive rate of CD166 expression in CNE-2 cell line was (24.27 ± 5.31)%. The positive rate of CD166 expression in CNE-2-CD166 (+) cells was 96.5% and that of CNE-2-CD166- The results of colony formation assay showed that the sensitization enhancement ratio (SER) of CNE-2-CD166 (-) cells to CNE-2-CD166 (+) cells was 1.24.10 Gy at 24 h, 48 h and The survival of CNE-2-CD166 (+) cells at 72 h were (76.66 ± 3.36)%, (62.44 ± 1.66)% and (55.21 ± 3.39)%, respectively ± 5.13%, (41.11 ± 3.00)% and (35.28 ± 4.36)%, respectively. The difference of survival score between the two groups was statistically significant (P <0.05). The apoptosis rates of CNE-2-CD166 (+) cells and CNE-2-CD166 (-) cells were (5.87 ± 1.14)% and (6.67 ± 1.68)% respectively before 10 Gy irradiation and the difference was not statistically significant > 0.05). The apoptotic rate of CNE-2-CD166 (+) cells was (19.37 ± 2.54)% at 48 h after 10 Gy irradiation and was significantly lower than that of CNE-2-CD166 (-) cells (28.90 ± 4.04) , The difference was statistically significant (P = 0.026). Conclusion CD166-positive nasopharyngeal carcinoma CNE-2 cells are more resistant to radiation, which may be related to the decrease of the apoptosis rate of cells after irradiation.