论文部分内容阅读
为了解重庆小麦的品质遗传基础,采用SDS-PAGE技术对重庆小麦推广品种和主要育种亲本共35份材料的HMW-GS组成和变异进行了分析.结果表明:参试材料Glu-1位点具有较为丰富的遗传变异,并检测到14种亚基类型和19种亚基组合类型.Glu-A1位点检测到2种亚基(1,null),其中null占82.86%;Glu-B1位点检测到9种亚基(7+8,7+9,7,22,6+8,14+15,17+18,20,8),其中以7+8,7+9出现的频率最高;Glu-D1位点检测到3种亚基(2+12,5+10,4+12),其中5+10占57.14%;参试材料中检测到与烘烤品质相关的优质亚基.参试材料的品质评分在4~10分之间,平均分为6.49分.在重庆市的7个小麦推广品种中,检测到6种亚基组合类型,品质评分平均为6.86分.
In order to understand the genetic basis of quality of wheat in Chongqing, the composition and variation of HMW-GS in 35 materials of wheat-promoting varieties and main breeding parents in Chongqing were analyzed by SDS-PAGE. The results showed that Glu- More abundant genetic variation, and detected 14 subunit types and 19 subunit combinations.Glu-A1 locus detected two kinds of subunits (1, null), of which null accounted for 82.86%; Glu-B1 sites Nine subunits (7 + 8, 7 + 9, 7, 22, 6 + 8, 14 + 15, 17 + 18, 20 and 8) were detected, of which 7 + 8 and 7 + Three subunits (2 + 12, 5 + 10, 4 + 12) were detected at the Glu-D1 loci, of which 5 + 10 accounted for 57.14%. High quality subunits related to baking quality were detected in the tested materials. The quality score of the test materials was between 4 and 10, with an average of 6.49.Among the 7 wheat varieties popularized in Chongqing, 6 subunit combinations were detected with an average quality score of 6.86.