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目的 :研究三七皂苷中R1、Rg1在正常和脑缺血再灌状态下大鼠体内的药代动力学变化。方法 :正常组大鼠股静脉注射三七皂苷 (5 0mg/kg) ,缺血再灌组大鼠阻断双侧颈总动脉造成不完全脑缺血 3 0min、复灌同时股静脉注射等量药物。采用反相HPLC法测定iv后不同时间点大鼠血浆中R1、Rg1浓度 ,计算其动力学参数。结果 :在正常和脑缺血再灌状态下 ,R1、Rg1动力学呈一室开放模型。正常状态下 ,R1和Rg1的t1/ 2 、AUC0～∞ 、CL(s)、Vd分别为 2 1 6± 7 9和 2 0 8± 3 7min、418 7± 85 9和 1 2 0±0 18mg/kg ;脑缺血再灌状态下 ,R1和Rg1的t1/ 2 、AUC0～∞ 、CL(s)、Vd分别为 19 2± 5 0和 2 0 0± 5 2min、416 7± 5 8 9和 13 68 0± 186 3 μg/ml·min、0 13 90± 0 0 413和 0 0 3 80± 0 0 0 60mg/kg·min、3 3 1± 0 5 4和 1 0 4± 0 15mg/kg。 结论 :在正常和脑缺血再灌状态下 ,R1、Rg1各自的动力学参数没有明显差异。R1、Rg1的动力学过程相似
Objective : To study the pharmacokinetics of R1 and Rg1 in Sanqi saponins in normal and cerebral ischemia reperfusion rats. METHODS: Rats in the normal group were injected with notoginsenoside (50 mg/kg) in the femoral vein. Rats in the ischemic reperfusion group were inactivated by bilateral common carotid arteries to cause incomplete cerebral ischaemia for 30 min and re-irrigation at the same time. drug. The concentrations of R1 and Rg1 in rat plasma at different time points after iv were measured by reversed-phase HPLC and the kinetic parameters were calculated. Results: Under normal and cerebral ischemia reperfusion conditions, the kinetics of R1 and Rg1 presented a one-compartment open model. Under normal conditions, the t1/2, AUC0~∞, CL(s), and Vd of R1 and Rg1 are 2 1 6±7 9 and 2 0 8±3 7min, 418 7± 85 9 and 120±0 18 mg, respectively. In the state of cerebral ischemia and reperfusion, the t1/2, AUC0-∞, CL(s), and Vd of R1 and Rg1 were 19 2±50 and 200±52 in, and 416 7±5 8 9 respectively. And 13 68 0± 186 3 μg/ml·min, 0 13 90± 0 0 413 and 0 0 80 ± 0 60 mg/kg·min, 3 3 1± 0 5 4 and 10 4± 0 15 mg/ Kg. Conclusion : Under normal and cerebral ischemia reperfusion conditions, the kinetic parameters of R1 and Rg1 were not significantly different. The kinetic processes of R1 and Rg1 are similar