本文作者采用免疫组织化学和生化方法检查重度慢性(特发性)便秘患者的乙状结肠标本内5-羟色胺(5-HT)和多巴胺β-羟化酶(DBH)分布,并与结直肠癌患者作比较。 9例原因不明的重度便秘患者(女8例,男1例,平均年龄37岁)进行部分或全结肠切除手术治疗时取乙状结肠组织进行检测。术前经钡灌肠检查示正常。另设对照组:14例(女5例,男8例,平均年龄54岁)结肠或直肠癌患者术时取远离肿瘤部位而肉眼正常的乙状结肠标本进行检测。术前排便次数正常且无梗阻症象。标本分成三部分进行检测,(1)粘膜层(MUC);(2)纵行肌、环状肌及肠肌丛和粘膜下丛(MP);(3)环形平滑肌层(CM)。结果:显微镜检查两组组织形态学表现无差异。免疫组织化学检查发现两组在MP内有5-HT免疫活性的神经纤维,CM内免疫活性神经纤维则稀少。5-HT免疫活性样神经支配的类型与以往的报道相似。MUC的肠嗜铬细胞内有强5-HT免疫荧光。虽DBH免疫荧光密度一般低于5-HT,但肌层和神经丛内DBH与5-HT免疫活性相似。免疫组织化学染色时粘膜层嗜铬 The authors examined the distribution of serotonin (5-HT) and dopamine β-hydroxylase (DBH) in sigmoid colon specimens of patients with severe chronic idiopathic constipation by immunohistochemistry and biochemical methods and compared them with those of patients with colorectal cancer Compare Nine cases of unresectable patients with severe constipation (8 males and 1 females, mean age 37 years) underwent partial or total colon resection surgery to take sigmoid colon tissue for testing. Preoperative barium enema showed normal. Another control group: 14 patients (5 females, 8 males, mean age 54 years) colon or rectal cancer patients were taken away from the tumor site and the naked eye sigmoid colon specimens were detected. Preoperative bowel movements normal and no obstruction symptoms. The specimens were divided into three sections for testing: (1) mucosal layer (MUC); (2) longitudinal muscle, circular muscle and myenteric plexus and submucosal plexus (MP); and (3) annular smooth muscle layer (CM). Results: Microscopy showed no difference in histological appearance between the two groups. Immunohistochemistry revealed that both groups had 5-HT immunoreactive nerve fibers in the MP, but few immunoreactive nerve fibers in the CM. The type of 5-HT immunoreactive-like innervation is similar to previous reports. Intestinal Chromaffin cells in MUC have strong 5-HT immunofluorescence. Although the density of DBH immunofluorescence is generally lower than that of 5-HT, DBH is similar to 5-HT in muscle and nerve plexus. Immunohistochemical staining of chromaffin mucosa