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目的研究炎症性肠病(Inflammatory Bowel Disease,IBD)患者肠黏膜髓系细胞表达触发受体-1(TREM-1)与环氧化酶-2(COX-2)的表达,以及两者与溃疡性结肠炎患者病情严重程度和内镜分级程度的关系。方法分别采用流式细胞技术和免疫组化方法对60例IBD患者和30例对照组患者肠黏膜中TREM-1和COX-2的表达进行测定,其中60例IBD患者中,包括52例溃疡性结肠炎(Ulcerative Colitis,UC)患者,8例克罗恩病(Crohn’s Disease,CD)患者。结果 (1)在对照组、克罗恩病和溃疡性结肠炎中COX-2的表达分别为:(61.81±20.10),(209.28±9.62)和(200.42±20.33),且对照组与克罗恩病和溃疡性结肠炎组相比,差异有统计学意义(P<0.05);TREM-1在3组中的表达分别为(268.94±4.75),(541.48±153.63)和(487.54±141.59),差异有统计学意义(P<0.05)。TREM-1在UC不同的病情分级及不同内镜分级中表达分别为:病情轻度:(449.55±49.45),中度(499.41±10.81),重度(507.49±67.79),差异有统计学意义(F=19.055,P<0.05);不同内镜分级中的表达是I级:(361.47±55.03),Ⅱ级(481.44±24.28),Ⅲ级(555.01±37.65),差异有统计学意义(F=34.119,P<0.05);COX-2在UC不同的病情分级及不同内镜分级中表达分别为:病情轻度(158.54±15.27),中度(195.25±10.10),重度(233.72±11.43),差异有统计学意义(F=54.617,P<0.05);不同内镜分级中的表达是:Ⅰ级(148.26±16.08),Ⅱ级(203.85±15.43),Ⅲ级(236.35±10.63),差异有统计学意义(F=58.608,P<0.05)。(2)IBD患者结肠黏膜中TREM-1和COX-2的表达呈正相关(r=0.642,P<0.05);UC患者结肠黏膜中TREM-1的表达水平与患者病情程度及内镜分级呈正相关(r=0.945,P<0.05;r=0.944,P<0.05),COX-2的表达水平与患者病情程度及内镜分级亦呈正相关(r=0.944,P<0.05;r=0.944,P<0.05)。结论 TREM-1和COX-2参与了IBD的发病过程,并且可以反映UC的炎症活动情况。
Objective To investigate the expression of TREM-1 and COX-2 in intestinal mucosa of patients with inflammatory bowel disease (IBD) The relationship between the severity of patients with colitis and the degree of endoscopic grading. Methods The expressions of TREM-1 and COX-2 in intestinal mucosa of 60 patients with IBD and 30 controls were measured by flow cytometry and immunohistochemistry. Among 60 IBD patients, including 52 cases of ulcerative Eight patients with Crohn’s Disease (CD) were enrolled in this study. Results (1) The expression of COX-2 in control group, Crohn’s disease and ulcerative colitis were (61.81 ± 20.10), (209.28 ± 9.62) and (200.42 ± 20.33), respectively. The expression of TREM-1 in the three groups were (268.94 ± 4.75), (541.48 ± 153.63) and (487.54 ± 141.59), respectively, compared with the ulcerative colitis group (P <0.05) , The difference was statistically significant (P <0.05). The expression of TREM-1 in different grades and different endoscopic grades of UC were mild (449.55 ± 49.45), moderate (499.41 ± 10.81) and severe (507.49 ± 67.79), respectively, and the difference was statistically significant ( F = 19.055, P <0.05). The expression of different endoscopic grades was grade I (361.47 ± 55.03), grade Ⅱ (481.44 ± 24.28), grade Ⅲ (555.01 ± 37.65), the difference was statistically significant (F = 34.119, P <0.05). The expression of COX-2 in different grades of UC and different endoscopic grades were mild (158.54 ± 15.27), moderate (195.25 ± 10.10), severe (233.72 ± 11.43) The difference was statistically significant (F = 54.617, P <0.05). The expressions of different endoscopic grades were: grade Ⅰ (148.26 ± 16.08), grade Ⅱ (203.85 ± 15.43) and grade Ⅲ (236.35 ± 10.63) Statistical significance (F = 58.608, P <0.05). (2) The expression of TREM-1 and COX-2 in colonic mucosa of IBD patients was positively correlated (r = 0.642, P <0.05). The expression of TREM-1 in colonic mucosa of UC patients was positively correlated with the degree of disease and the endoscopic grade (r = 0.945, P <0.05; r = 0.944, P <0.05). There was also a positive correlation between the expression of COX-2 and the severity of disease and endoscopic grade (r = 0.944, 0.05). Conclusion TREM-1 and COX-2 are involved in the pathogenesis of IBD and can reflect the inflammatory activity of UC.