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目的研究nm23H1mRNA表达水平与食道癌细胞转移倾向的相关性。方法活检标本采用二次包埋法制备连续冰冻切片,应用地高辛标记的nm23H1反意cRNA探针进行原位杂交并做定位分析。结果原位杂交染色阳性者为胞浆型颗粒状或团块状。在50例活检标本中nm23H1mRNA表达阳性率为38%。在食道癌长度>5cm的17例中有3例阳性,在术后病理检查21例局部淋巴结转移者中阳性表达3例。结论nm23H1mRNA表达水平与食道癌转移倾向呈负相关(P<005)。
Objective To investigate the correlation between the expression of nm23H1 mRNA and the metastasis tendency of esophageal cancer cells. Methods The biopsy specimens were prepared by secondary embedding and frozen sections were prepared. The digoxigenin-labeled nm23H1 antisense cRNA probe was used for in situ hybridization and localization analysis. Results In situ hybridization staining was positive for cytoplasmic granular or lumps. The positive rate of nm23H1 mRNA expression was 38% in 50 biopsy specimens. Of the 17 patients with esophageal cancer >5 cm, 3 were positive, and 3 were positive in 21 patients with regional lymph node metastasis after pathological examination. Conclusion The expression of nm23H1 mRNA is negatively correlated with the metastasis tendency of esophageal cancer (P<005).