用含有致死剂量的蜕皮抑制剂灭幼脲I号(Dimilin)饲料喂养落叶松毛虫幼虫,幼虫内部组织的中肠及马氏管未出现变化,体壁出现一些变化。显微切片观察,内表皮组织呈现损伤,不是完全附着在上表皮上,出现疏松分离状态。同时进一步对体壁定量化学分析表明,内表皮中的几丁质沉积量约减少了12％;而另一方面,蛋白质的含量不受影响。这就揭示了灭幼脲I号对松毛虫有一种特殊的作用方式——妨碍几丁质的沉积,使新形成的表皮缺少硬度,在蜕皮期间不能正常蜕掉旧皮最终导致死亡。从此证明灭幼脲T号和常规杀虫剂性质不同,能引起松毛虫在蜕皮期间死亡,如剂量较低,可使发育迟缓或畸形。 Larvae were inoculated with the lethal dose of the molting inhibitor Dimilin. The larvae did not show any changes in the midgut and midgut of the larvae. Micro-section observation, the inner epidermis tissue injury, not completely attached to the epidermis, loose separation. At the same time, further quantitative analysis of the body wall showed that the amount of chitin deposition in the inner epidermis was reduced by about 12%; on the other hand, the protein content was not affected. This revealed that diflubenzuron I had a special mode of action on the pine caterpillars - obstructing the deposition of chitin, lacking the hardness of the newly formed epidermis, and eventually losing the skin during molting, eventually leading to death. From this it was demonstrated that the diflubenzuron differed in properties from the conventional insecticide, causing the pine caterpillars to die during molting, such as lower doses, which could retard growth or deformity.