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【目的】不同植物生长调节物质对植食性昆虫产卵、发育、取食等常有促进或抑制作用,进而影响植物与昆虫的互作关系。本研究旨在阐明常用的植物生长调节剂吲哚-3-乙酸对B型烟粉虱Bemisia tabaci(Gennadius)的影响,进而探索防治烟粉虱环境友好的方法,同时期望获得更加优质的烟粉虱用作替代寄主饲养寄生蜂。【方法】对番茄Solanum lycopersicum L.植株外源喷施吲哚-3-乙酸(IAA),检测其生理生化的响应;记录B型烟粉虱存活率、产卵量并测量解毒酶等指标。【结果】喷施IAA后番茄叶片含水量略有上升,叶面积增大,叶绿素含量增加,过氧化物酶(POD)活性降低,多酚氧化酶(PPO)活性升高,超氧化物歧化酶(SOD)活性变化不显著(P>0.05)。喷施IAA后番茄上B型烟粉虱成虫存活率高于对照组,其中50 mg/L IAA番茄上B型烟粉虱成虫存活率和总产卵量最高,250mg/L IAA存活率最低;喷施IAA 120 h后烟粉虱总产卵量升高;碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)活性较对照升高。【结论】喷施不同浓度的IAA溶液后,番茄植株的生理生化水平发生不同程度的改变,烟粉虱成虫的存活状况、产卵量以及体内解毒活性也会产生不同程度的改变。适宜剂量的IAA处理能够使烟粉虱的生存表现更加优异,从而为饲养寄生蜂提供优质的替代寄主。
【Objective】 Different plant growth regulators often promote or inhibit spawning, development and feeding of herbivorous insects, and then affect the interaction between plants and insects. The aim of this study was to elucidate the effect of indole-3-acetic acid, a commonly used plant growth regulator, on Bemisia tabaci (Gennadius), a biotype of Bemisia tabaci B (Gennadius), to explore environmentally friendly methods for control of Bemisia tabaci. Lice are used as surrogate hosts to feed parasitic wasps. 【Method】 To investigate the physiological and biochemical responses of solanum lycopersicum L. to IAA, the survival rate of B. tabaci B-biotype, the number of eggs laid and the detoxification enzymes were measured. 【Result】 The results showed that the water content of tomato leaves increased slightly after spraying IAA, the leaf area increased, the content of chlorophyll increased, the activity of peroxidase (POD) decreased, the activity of polyphenol oxidase (PPO) increased, the activity of superoxide dismutase (SOD) activity did not change significantly (P> 0.05). The survival rate of B. tabaci B-type on white tomato was higher than that of the control after spraying IAA. The survival rate and total fecundity of B. tabaci B were the highest at 50 mg / L IAA tomato, while the survival rate of IAA at 250 mg / L was the lowest. The total fecundity of B. tabaci increased after spraying IAA for 120 h. The activities of alkaline phosphatase (AKP) and superoxide dismutase (SOD) were higher than those of the control. 【Conclusion】 Physiological and biochemical changes of tomato plants changed to different extents after sprayed with different concentrations of IAA solution. The survival status, the amount of spawning and the detoxification activity of Bemisia tabaci adults also varied to some extent. Appropriate doses of IAA treatment can make the survival performance of Bemisia tabaci more excellent, so as to provide a good alternative host for feeding parasitoids.