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目的 :建立测定复方替硝唑搽剂中替硝唑和水杨酸含量的高效液相色谱法。方法 :采用反相ODS色谱柱 ,甲醇 - 0 0 2mol·L-1KH2 PO4 溶液 (5 0∶5 0 )为流动相 ,检测波长为 315nm。结果 :替硝唑和水杨酸的线性范围分别在5 0~ 35 0 μg·mL-1(r=0 9997) ,40 0~ 16 0 0 μg·mL-1(r =0 9997) ;加样回收率分别为 10 0 6 % (RSD =0 6 9% )及 10 0 6 % (RSD =0 79% ) ;日内RSD为 0 16 %~ 1 2 %和 0 6 1%~ 1 2 % ,n =12 ,日间RSD为 0 2 4%~ 1 7%和0 2 %~ 1 1% ,n =4。结论 :HPLC法可用于复方替硝唑搽剂的含量测定 ,方法准确、灵敏 ,专属性强
Objective: To establish a HPLC method for the determination of tinidazole and salicylic acid in compound tinidazole liniment. Methods: The reversed-phase ODS column was used. The mobile phase consisted of methanol-0.02 mol·L-1 KH2PO4 solution (50:50) and the detection wavelength was 315 nm. Results: The calibration curves of tinidazole and salicylic acid were linear over the range of 5 0-35 0 μg · mL-1 (r = 0 9997) and 40 0-16 0 0 μg · mL-1 (r = 0 9997) The average recoveries were 106% to 120% and 106% to 122% for RSD of 0 6 9% and 100 6% respectively (RSD = 79% n = 12, daytime RSD was 0 2 4% ~ 1 7% and 0 2% ~ 1 1%, n = 4. Conclusion: HPLC method can be used for the determination of compound tinidazole liniment, the method is accurate, sensitive and specific