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免疫-PCR结合了抗原抗体反应的特异性和PCR的高敏感性,是一种极为敏感的抗原检测技术,并适合于各种微量抗原的检测。免疫-PCR的关键在于形成抗体-标记DNA偶联物,作为桥梁连接免疫反应的特异性和PCR的高扩增能力。本文简述了免疫-PCR的基本流程以及抗体与标记DNA相偶联、PCR产物检测方法的进展。
Immuno-PCR combines the specificity of antigen-antibody reaction with the high sensitivity of PCR. It is an extremely sensitive antigen detection technique and suitable for the detection of various trace antigens. The key to immune-PCR lies in the formation of antibody-labeled DNA conjugates, as a specificity of bridging-linked immune responses and high amplification of PCR. This article outlines the basic steps of immuno-PCR and the conjugation of antibodies to labeled DNA, and advances in the detection of PCR products.