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目的探讨钙和大豆异黄酮的联合作用对去卵巢大鼠骨生物力学特性的影响。方法将160只12月龄SD清洁级雌性大鼠随机分为4组,分别为假手术对照(蒸馏水)组、骨质疏松模型(蒸馏水)组、大豆异黄酮(10 mg/ml,染毒容量为3 ml/kg)组、雌激素(0.1 mg/ml,染毒容量为0.1 ml/kg)组,每组再按照加钙(150 mg/ml,染毒容量为1 ml/d)和不加钙(蒸馏水)分为两组,每组20只。连续染毒6个月。分别在染毒3和6个月时,测量股骨的6项生物力学指标(扭转刚度、柔韧系数、最大载荷、破坏扭转角、最大正应力、破坏能量吸收)并进行统计分析。结果染毒3个月,钙能显著提高大鼠股骨柔韧系数、最大载荷和破坏能量吸收的指标值;大豆异黄酮、钙与大豆异黄酮的交互作用能显著提高股骨扭转刚度、柔韧系数、最大载荷、最大正应力、破坏能量吸收5个生物力学指标值(对破坏扭转角影响不显著)。染毒6个月,钙、大豆异黄酮以及钙与大豆异黄酮的交互作用均能显著提高股骨6个生物力学指标的均值,与染毒3个月时相比,影响效果均显著增强(P<0.05)。结论钙与大豆异黄酮联合作用可以明显改善骨生物学性能。
Objective To investigate the effects of combined action of calcium and soy isoflavones on the biomechanical properties of ovariectomized rats. Methods 160 SD rats of 12 months of age were randomly divided into 4 groups: sham operation control group (distilled water group), osteoporosis model group (distilled water group), soy isoflavone (10 mg / ml, (3 ml / kg) and estrogen (0.1 mg / ml, 0.1 ml / kg). Each group was treated with 150 mg / ml calcium and 1 ml / Calcium (distilled water) is divided into two groups of 20 each. Continuous exposure for 6 months. Six biomechanical parameters of the femur (torsion stiffness, flexural coefficient, maximum load, breaking torsion angle, maximum normal stress, energy absorption under destruction) were measured at 3 and 6 months of exposure respectively and statistically analyzed. Results Calcium could significantly increase the index of flexibility, maximal load and energy absorption of damaged femur after 3 months of exposure. Interaction of soy isoflavones and calcium with soy isoflavones could significantly increase the torsional stiffness and flexibility of the femur, Load, the maximum normal stress, damage energy absorption of five biomechanical indicators (damage to the torsion angle is not significant). Six months after exposure, the interaction between calcium, soy isoflavones and calcium and soy isoflavone significantly increased the mean of 6 biomechanical indexes of femur, which were significantly higher than those at 3 months (P <0.05). Conclusion The combined effect of calcium and soy isoflavones can significantly improve the biological performance of bone.