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目的预测EB病毒潜伏膜蛋白1(Latent Membrane Protein1,LMP1)的B细胞表位。方法基于EB病毒基因组序列,采用DNAStar Lasergene软件包中的Protean软件,对LMP1的亲水性,表面可能性,抗原指数及其二级结构中的柔性区域进行分析,并结合吴玉章的抗原指数预测法预测其B细胞表位。结果 B细胞表位最有可能位于潜伏膜蛋白N端第356-358,2-19,249-314区段或其附近,而潜伏膜蛋白N端第185-223区段内或附近也可能存在B细胞表位。结论用多参数预测EB病毒LMP1的B细胞表位,为鼻咽癌的筛查及抗肿瘤转移靶向治疗的分子免疫学研究奠定基础。
Objective To predict the B cell epitope of Latent Membrane Protein 1 (LMP1). Methods Based on the Epstein-Barr virus genome sequence, Proteam software in DNAStar Lasergene software package was used to analyze the hydrophilicity, surface potential, antigenic index and the flexible region in the secondary structure of LMP1, and combined with Wu Yuzhang’s antigenic index prediction Predict their B cell epitopes. Results B-cell epitopes were most likely to be located at or near the N-terminal 356-358, 2-19, 249-314 of the latent membrane protein while B cells could also be present in or near the N-terminal 185-223 segment of the latent membrane protein gauge. Conclusion The multiple parameter prediction of B cell epitopes of Epstein-Barr virus LMP1 lays the foundation for the screening of nasopharyngeal carcinoma and the molecular immunological study of anti-tumor metastasis targeting therapy.