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目的探讨TBX21基因T-1993C多态性对T-bet表达及Ⅰ类辅助性T细胞极化群体的影响。方法通过EMSA实验观察TBX21启动子区-1993位点顺式调控作用,采用PCR-RFLP方法对2010年1月10日至20日我院体检中心收集的370例健康献血员[男性206例,女性110例,年龄21～57(36±9.2)岁]全血样本进行TBX21-1993位点基因分型,应用流式细胞仪检测不同基因型个体T-bet及Th1极化相关细胞因子IFN-γ表达水平。结果 TBX21基因-1993位点T/C等位探针均与一未知核因子发生特异性结合,且与C等位亲和力明显高于T等位;不同基因型个体T-bet及Th1极化相关细胞因子IFN-γ表达由TT→TC→CC基因型呈递减趋势,差异显著(P<0.05)。结论 TBX21基因-1993位点是一个重要的功能位点,T/C等位均与一未知核转录因子特异性结合并负性调控T-bet及Th1极化相关细胞因子IFN-γ表达,从而反馈性调节TH1分化。
Objective To investigate the effect of T-1993C polymorphism of TBX21 gene on the expression of T-bet and the type Ⅰ helper T cell polarization population. Methods EMSA was used to observe the cis-regulatory role of -1993 at the promoter region of TBX21. PCR-RFLP was used to analyze the frequency of 370 healthy blood donors collected from the physical examination center of our hospital from January 10 to January 20,2010 [206 males 110 cases of age ranged from 21 to 57 years (36 ± 9.2) years of age were genotyped for TBX21-1993 loci. Flow cytometry was used to detect T-bet and Th1-related cytokine IFN-γ in different genotypes The expression level. Results The T / C allele at -1993 site of TBX21 gene specifically bound to an unknown nuclear factor, and the allele affinity with C was significantly higher than that of T allele. The T-bet and Th1 polarization of TBX21 gene- The expression of cytokine IFN-γ showed a decreasing trend from TT → TC → CC genotype (P <0.05). Conclusion The -1993 site of TBX21 gene is an important functional site. The T / C allele specifically binds to an unknown nuclear transcription factor and negatively regulates the expression of T-bet and Th1-related cytokine IFN-γ Feedback regulates TH1 differentiation.