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用羧甲基化的N,N’-甲叉双丙烯酰胺交联的烯丙基葡聚糖(简称CM-CADB)凝胶树脂为新型载体,研究了谷氨酸脱羧酶(GDC)在CM-CADB上的固定化与环境的依赖关系.确定了酶固定化最佳条件,研究了固定化GDC的活性与底物浓度、pH、温度的依存性,动态响应特性,热稳定性和寿命,求算了米氏常数.将固定化GDC酶柱与进样系统具CO_2气敏电极的离子活度分析器-计算机数据采集系统匹配,构成酶反应谷氨酸检测装置,测定了固定化GDC酶柱的能斯特线性响应曲线,线性回归方程为y=43.3x+181.6,r为0.9936.
A novel carrier of allyl dextran (CM-CADB) gel resin crosslinked with carboxymethylated N, N’-methylene bisacrylamide was used to study the effect of glutamate decarboxylase (GDC) -CADB immobilization and environmental dependence.Determined the best conditions for enzyme immobilization, immobilized GDC activity and substrate concentration, pH, temperature dependence, dynamic response characteristics, thermal stability and longevity, Calculated the Michaelis constant.The immobilized GDC enzyme column and the injection system with CO_2 gas sensor ion activity analyzer - computer data acquisition system to match, constitute the enzyme reaction glutamate detection device was measured immobilized GDC enzyme Nernst linear response curve of the column, the linear regression equation is y = 43.3x + 181.6, r is 0.9936.