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目的观察昆明小鼠感染曼氏裂头蚴后组织中裂头蚴的寄生情况及血清中IgG抗体含量的动态变化,以了解昆明小鼠用于曼氏裂头蚴感染模型建立的价值。方法从黑斑蛙体内检获曼氏裂头蚴,口服法5条/只感染昆明小鼠,于感染后第2～10周分批处死小鼠,进行大体观察,并用间接ELISA法检测血清中特异性IgG抗体的水平。结果小鼠感染裂头蚴后,多组织器官有裂头蚴寄生,其中以皮下肌肉处多见。裂头蚴寄生处组织有弥漫性淤血斑点或囊包块形成。实验小鼠血清中特异性IgG抗体于感染后第2周检出,第2～8周呈上升趋势,第8周达峰值,后逐渐下降。结论昆明小鼠感染曼氏裂头蚴后组织中可检出裂头蚴和特异性抗体,用于建立曼氏裂头蚴感染模型,成功率高、造模简单、经济适用。
Objective To observe the parasitic status of sporozoite and the content of IgG in serum of Kunming mouse infected with Spirillard’s larvae of Kunming in order to understand the value of establishing Kunming mouse model of sporozoite infection. Methods Manfist larvae were collected from the black spot frog body. Five mice were infected with Kunming mice by oral route. The mice were sacrificed at the second to tenth weeks after infection. The mice were sacrificed in batches and the serum levels of specific IgG Antibody levels. Results In mice infected with the larvae, many tissues and organs have parasitic sporozoites, of which subcutaneous muscles more common. Sporozoite parasite organizations have diffuse congestion spots or capsule mass formation. The specific IgG antibodies in the serum of the experimental mice were detected at the second week after infection, increasing from the second week to the eighth week and reaching the peak on the eighth week, and then gradually decreased. Conclusion Kunming mouse infected with Manifesting Schizosaccha was in the organization can detect sporozoite and specific antibodies for the establishment of Mann’s infection of sparganosis with high success rate, simple model, economical and affordable.