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目的采用瘤细胞悬液和瘤组织块原位种植构建Sprague Dawley(SD)大鼠原位移植胃癌模型,并比较其诱癌率。方法分别采用直接注射法和组织插块法构建SD大鼠胃癌模型:直接注射法组将walker-256细胞悬液直接注射到SD大鼠胃壁的浆膜内,观察移植肿瘤的生长状况、移植成功率;组织插块法组以walker-256细胞接种于SD幼鼠皮下,形成稳定传代的皮下移植瘤,再取该肿瘤组织块原位移植于SD大鼠胃壁,观察移植肿瘤的生长状况、移植成功率。结果直接注射法组原位移植成功率为5/7,造模9 d后平均瘤重为0.320 g;组织插块法组原位移植胃癌成功率为3/7,造模9 d后平均瘤重为0.206g。直接注射法组原位移植成功率和造模9 d后平均瘤重均较组织插块法组高。结论直接注射法原位移植模型的生物学行为与人胃癌自然生长过程相似,可作为一种有价值的工具用于胃癌转移机制和抗转移实验。
Objective To establish a model of orthotopic implantation of gastric cancer in Sprague Dawley (SD) rats by in situ implantation of tumor cell suspension and tumor mass. Methods The SD rat model of gastric cancer was established by direct injection and tissue insertion respectively. The direct injection of walker-256 cell suspension was injected directly into the serosal wall of SD rats, and the growth of the tumor was observed. Rate. Tissue plug method group was inoculated by walker-256 cells subcutaneously in SD rats to form a stable subcultured xenograft tumor. The tumor tissue pieces were transplanted into the stomach of SD rats in situ and the growth of transplanted tumor was observed. Success rate. Results The success rate of orthotopic implantation was 5/7 in the direct injection group and 0.320 g in the 9th day after modeling. The successful rate of orthotopic implantation of gastric cancer in the tissue insertion group was 3/7, Weight 0.206g. The success rate of orthotopic transplantation in direct injection group and the average tumor weight after 9 days of modeling were higher than that of tissue insertion group. Conclusion The biological behavior of orthotopic transplantation in situ injection is similar to the natural growth of human gastric cancer. It may be used as a valuable tool for gastric cancer metastasis and anti-metastasis.