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单独采用茎尖嫁接法和单独采用热处理法均难脱除温州蜜柑萎缩病毒(SDV),但采用白天40℃光照,夜间30℃黑暗(各12小时)热处量7～43天,结合茎尖嫁接法获得的72株茎尖苗全部脱除SDV。所以采用上述方法热处理7天,结合茎尖嫁接是脱除SDV的简便可靠的方法。
It is difficult to remove the satsuma wilt virus (SDV) by stem-tip grafting and heat-treatment alone, but heat treatment is performed at 40 ℃ in the day and at night in the dark at 30 ℃ for 12 to 43 days respectively. The 72 shoot-tip shoots obtained by grafting all removed SDV. Therefore, the heat treatment using the above method for 7 days, combined with the shoot tip grafting SDV is a simple and reliable method.