当血吸虫成虫降解利用血红蛋白时,蛋白酶起着重要作用,已报道血吸虫有与哺乳动物组织蛋白酶B和L型同源的蛋白酶,曼氏血吸虫体内存在组织蛋白酶类B型(Sm31),它是一种重要的诊断抗原,亦可作为化疗的靶物质。通常采用传统的层析法或分子生物学方法分离提取血吸虫蛋白酶,本文作者采用亲合层析法,首次从日本血吸虫成虫分离纯化出组织蛋白酶类B型(Sj31),同时对Sj31作生化特征分析和组织定位研究。 Protease plays an important role when adult schistosomiasis degrades and uses hemoglobin. It has been reported that Schistosoma japonicum has a protease homologous to mammalian cathepsin B and L, and cathepsin B (Sm31) is present in Schistosoma mansoni Important diagnostic antigen, but also as a target of chemotherapy. Schistosoma japonicum protease is usually isolated and purified by traditional methods of chromatography or molecular biology. The authors used affinity chromatography to isolate and purify cathepsin B (Sj31) from Schistosoma japonicum for the first time, and biochemically analyzed Sj31 And tissue location studies.