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目的探讨人类白细胞抗原HLA-DRB1等位基因多态性与新疆宫颈癌高发区喀什维吾尔族妇女宫颈癌的关系。方法采用聚合酶联反应序列特异性寡核苷酸探针(polymerase chain reaction sequence-specificoligonucleotide,PCR-SSO)法检测90例新疆喀什维吾尔族宫颈癌患者及90例当地正常妇女宫颈组织中HLA-DRB1的等位基因。结果等位基因HLA-DRB1*15在宫颈癌组中出现的频率明显高于对照组,差异有统计学意义(χ2=4.05,P<0.05,OR=2.002),表明HLA-DRB1*15可能是喀什维吾尔族妇女宫颈癌的易感基因;而等位基因HLA-DRB1*04在宫颈癌组中出现的频率明显低于对照组,差异有统计学意义(χ2=7.283,P<0.05,OR=0.432),表明HLA-DRB1*04可能是喀什维吾尔族妇女宫颈癌的保护基因;HLA-DRB1的其他等位基因在两组中出现的频率差异无统计学意义(P>0.05)。结论 HLA-DRB1*15可能与新疆宫颈癌高发区喀什维吾尔族妇女对宫颈癌的遗传易感性有关,是当地维吾尔族妇女宫颈癌的易感基因;而HLA-DRB1*04可能是喀什维吾尔族妇女宫颈癌的保护基因。两组基因的检测在维族妇女高危人群的筛选及群体遗传干预工作中有重要意义。
Objective To investigate the relationship between human leukocyte antigen HLA-DRB1 alleles and cervical cancer in Uighur women in Kashgar in high incidence area of cervical cancer in Xinjiang. Methods HLA-DRB1 was detected in 90 cases of cervical cancer in Kashgar Uighur Xinjiang and 90 cases of local normal women by polymerase chain reaction sequence-specific oligonucleotide (PCR-SSO) Of alleles. Results The frequency of allele HLA-DRB1 * 15 in cervical cancer group was significantly higher than that in control group (χ2 = 4.05, P <0.05, OR = 2.002), indicating that HLA-DRB1 * 15 may be The frequencies of allele HLA-DRB1 * 04 in cervical cancer group were significantly lower than those in control group (χ2 = 7.283, P <0.05, OR = 0.432), indicating that HLA-DRB1 * 04 may be a protective gene for cervical cancer in Kashgar Uighur women. There was no significant difference in the frequencies of other HLA-DRB1 alleles between the two groups (P> 0.05). Conclusions HLA-DRB1 * 15 may be related to the genetic susceptibility of cervical cancer to Uighur women in Kashgar in Xinjiang Uighur. It may be a susceptible gene for Uighur women with cervical cancer. HLA-DRB1 * 04 may be a Uighur woman in Kashgar Cervical cancer protection gene. The detection of two groups of genes is of great significance in the screening of high-risk Uyghur women and population genetic intervention.