针对荧光成像仪氙灯光源寿命短,激发能力弱的现状,提出了运用超连续谱激光(SCL)作为荧光激发光源的生物荧光成像技术;进行了荧光标准测试卡在两种光源激发下的荧光对比实验和不同SCL激发功率谱密度下的分析实验;通过线性拟合和外推的方法,对比分析了在相同曝光时间和功率下,两种光源的荧光激发能力;最后,通过对荧光基团在两种光源下的平均、最大光子密度流信息进行分析,进一步验证了实验结论:在生物荧光成像技术中,在相同激发功率谱密度下,相同的曝光时间内,SCL光源所激发的荧光强度是氙灯光源的2倍,且SCL具有更长的光源寿命和连续可调的光源参数,可满足荧光成像研究过程中对光源参数的不同需求,所做工作有利于解决荧光成像技术的光源瓶颈。 In view of the fact that the xenon light source of the fluorescence imager has a short lifetime and weak excitation capability, a biological fluorescence imaging technique using a supercontinuum laser (SCL) as a fluorescence excitation light source is proposed. Fluorescence contrast of a fluorescence standard test card excited by two light sources Experiments and different SCL excitation power spectral density analysis experiments; by linear fitting and extrapolation methods, comparative analysis of the same exposure time and power, two kinds of light source fluorescence excitation ability; finally, by the fluorescence group in the Two kinds of light sources under the average, maximum photon density flow information analysis, to further verify the experimental conclusion: In the biological fluorescence imaging technology, the same excitation power spectral density, the same exposure time, SCL light source excitation fluorescence intensity Xenon lamp, and SCL has longer lamp life and continuously adjustable light source parameters to meet the different needs of light source parameters in the process of fluorescence imaging research. The work is helpful to solve the light source bottleneck of fluorescence imaging technology.