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目的观察游泳训练对胰岛素抵抗大鼠肝脏抵抗素mRNA表达的影响,初步探讨游泳训练改善胰岛素抵抗的作用机制。方法 30只8周龄正常雄性大鼠随机分为正常饮食对照组(n=9)和高脂高糖喂养造模组(n=21)。喂养6周后,将造模成功的18只胰岛素抵抗大鼠再随机分为模型组(n=9):继续予高脂高糖饮食;运动组(n=9):高脂高糖喂养加游泳训练。造模6周和干预6周后采血清全自动生化分析仪测空腹血糖(FPG),放射免疫法测各组小鼠空腹胰岛素(FIN),计算胰岛素敏感指数(ISI)。干预6周后留取肝脏组织RT-PCR法检测抵抗素mRNA表达水平。结果高脂高糖饲养6周后,造模组的FPG(6.65±0.98)、FIN(31.04±6.57)均高于对照组,其ISI低于对照组(P<0.01)。6周干预后,与模型组比较,运动组肝脏组织抵抗素mRNA表达均降低(P<0.05),ISI升高(P<0.05)。结论游泳训练改善胰岛素抵抗可能与下调抵抗素的表达有关。
Objective To observe the effect of swimming training on the expression of resistin mRNA in rats with insulin resistance and to explore the mechanism of swimming training in improving insulin resistance. Methods Thirty normal 8-week-old male rats were randomly divided into normal diet control group (n = 9) and high fat and high glucose feeding group (n = 21). After 6 weeks of feeding, 18 rats with insulin resistance were randomly divided into model group (n = 9): continuous high-fat and high-sugar diet; exercise group (n = 9) Swimming training. After 6 weeks of intervention and 6 weeks of intervention, fasting blood glucose (FPG) was measured by automatic biochemical analyzer, and fasting insulin (FIN) was measured by radioimmunoassay. Insulin sensitivity index (ISI) was calculated. Six weeks after the intervention, the liver tissue was collected for RT-PCR to detect the expression of resistin mRNA. Results FPG (6.65 ± 0.98) and FIN (31.04 ± 6.57) in model group after 6 weeks of feeding were higher than those in control group, and the ISI of model group was lower than that of control group (P <0.01). Compared with model group, expression of resistin mRNA in liver tissue of exercise group decreased (P <0.05) and ISI increased (P <0.05) after 6 weeks’ intervention. Conclusion Swimming training to improve insulin resistance may be related to down-regulating the expression of resistin.