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本文作者选用纯种Wistar大白鼠,雌雄不限,于腹腔内注射2 ml左右0.5%巴比妥盐水作麻醉,随即剖开胸腹腔进行实验。本文报告了几种常用冷冻割断法用于肝肾等实质脏器扫描电镜标本制作的结果。研究表明:扫描电镜自备冷冻割断法简便,但标本易形成冰晶,不宜长期反复观察;田中氏法能显示细胞的细微结构,但操作较烦琐,重复性较低,且对肝肾等组织内部腔隙结构显示不佳;酒精割断法虽然对细胞内
The authors selected purebred Wistar rats, both male and female, intraperitoneal injection of about 2 ml of 0.5% barbiturate saline for anesthesia, then opened the chest and abdomen experiments. This article reports the results of several commonly used cryosection methods for the production of SEM and other real organs such as liver and kidney. The research shows that SEM is easy to cut by freezing, but the specimen is easy to form ice crystals, which is not suitable for long-term repeated observation. Tanaka’s method can show the fine structure of cells, but the operation is cumbersome and less reproducible, Lamellar structure shows poorly; alcohol severing method though intracellular