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目的:探讨炎性细胞因子肿瘤坏死因子-α、白细胞介素-6在糖尿病大鼠骨骼肌中的表达及其与细胞凋亡的关系。方法:将16只Wistar大鼠随机分为实验组8只与对照组8只。实验组喂养8周高脂高糖饮食空腹12 h后腹腔内注射小剂量链脲佐菌素(30 mg/kg),对照组喂养标准饲料8周空腹12 h后腹腔内注射柠檬酸-柠檬酸钠缓冲液,每4周测体质量、血糖。第13周末处死大鼠,取大鼠股四头肌,用免疫组织化学方法检测肿瘤坏死因子-α、白细胞介素-6及细胞凋亡蛋白bcl-2表达情况,并分析其相关性。结果:实验组大鼠骨骼肌中肿瘤坏死因子-α与白细胞介素-6表达水平高于对照组(P<0.01),bcl-2表达低于对照组(P<0.01),肿瘤坏死因子-α、白细胞介素-6与bcl-2表达呈负相关(r=-0.876,r=-0.823,P<0.05)。结论:糖尿病骨骼肌病变时炎症与凋亡同时发生,并可能共同导致胰岛素抵抗。
Objective: To investigate the expression of inflammatory cytokines TNF-α and IL-6 in skeletal muscle of diabetic rats and its relationship with apoptosis. Methods: Sixteen Wistar rats were randomly divided into experimental group (n = 8) and control group (n = 8). The experimental group were fed with high-fat and high-sucrose diet for 8 weeks and then injected with low-dose streptozotocin (30 mg / kg) intraperitoneally 12 hours later. The control group were fed with standard diet for 8 weeks and then injected intraperitoneally with citric acid-citric acid Sodium buffer, body mass every 4 weeks, blood glucose. At the end of the thirteenth week, the rats were sacrificed and the quadriceps of rats were sacrificed. The expression of tumor necrosis factor-α, interleukin-6 and bcl-2 were detected by immunohistochemical method and the correlation was analyzed. Results: The expression of tumor necrosis factor - α and interleukin - 6 in skeletal muscle of experimental group was higher than that of control group (P <0.01), while the expression of bcl - 2 was lower than that of control group (P <0.01) α, interleukin-6 and bcl-2 were negatively correlated (r = -0.876, r = -0.823, P <0.05). CONCLUSION: Inflammation and apoptosis occur simultaneously in diabetic skeletal muscle and may lead to insulin resistance.