Chiodini在15.4％及46.2％的Crohn病人中检出副结核分支杆菌及胞壁缺乏细菌,故分枝杆菌是Crohn病病因的可能性又被重提。作者用相似的方法研究了22例Crohn病及22例其他结肠病病人的粘膜。手术时还切除2个肠系膜淋巴结及一片浆膜,但只40％标本用0.1％(w/v)氯化苄烷铵脱污染处理10分钟。平均培养14月(范围4.6～27.2月)后从12例Crohn病人所取17份组织的Herrold蛋黄培基斜面上检到小(0.5 Chiodini was detected in 15.4% of Crohn patients and 46.2% of patients with Mycobacterium paratuberculosis and cell wall lack of bacteria, so the possibility of mycobacteria is the cause of Crohn disease was re-raised. In a similar fashion, the authors studied the mucosa of 22 patients with Crohn’s disease and 22 patients with other colorectal diseases. Two mesenteric lymph nodes and a serosa were resected at the time of surgery, but only 40% of the specimens were decontaminated with 0.1% (w / v) benzalkonium chloride for 10 minutes. After a mean incubation of 14 months (range 4.6-2.7.2 months), 17 tissues from 12 Crohn patients were examined for a small (0.5