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目的：建立人尿中Ｒ，Ｓ美芬妥英的液相色谱手性分离与检测方法，用于ＣＹＰ２Ｃ１９酶活性的快速测定．方法：受试者口服消旋美芬妥英后的０－８ｈ尿液标本用二氯甲烷提取后用手性色谱柱进行分离，流动相为乙腈和水（１４∶８６，体积比），其中含有０．１％的冰醋酸和０．２％的三乙胺，流速为０．９ｍＬ·ｍｉｎ－１，紫外检测波长为２０７ｎｍ．结果：在选定的色谱条件下Ｒ，Ｓ美芬妥英能很好地分离，尿中其他物质无干扰．用外标法定量，线性范围在５０－５０００μｇ·Ｌ－１，最小检出浓度为１２．５μｇ·Ｌ－１，保留时间在９ｍｉｎ内．液相色谱分析结果和气相色谱分析具有良好的一致性．结论：该法样本制备简便、分析时间短、线性范围宽、干扰少、灵敏和准确，可用于人体内美芬妥英代谢的研究和肝药酶ＣＹＰ２Ｃ１９酶活性的检测
Objective: To establish a liquid chromatography chiral separation and detection method for R, S-mephenytoin in human urine for the rapid determination of CYP2C19 enzyme activity. Methods: The urine samples of 0-8h after meclofenoxaban oral administration were extracted with methylene chloride and separated by chiral column. The mobile phase consisted of acetonitrile and water (14:86, volume ratio), in which Containing 0.1% of glacial acetic acid and 0.2% of triethylamine, the flow rate was 0.9mL · min-1, UV detection wavelength of 207nm. Results: Under the selected chromatographic conditions, R, S-mephenytoin can be well separated, other substances in the urine without interference. Using external standard method, the linear range of 50-5000μg · L-1, the minimum detectable concentration of 12.5μg · L-1, retention time in 9min. Liquid chromatography and gas chromatography analysis of the results with good consistency. Conclusion: The method is simple and convenient to prepare, the analysis time is short, the linear range is wide, the interference is small, sensitive and accurate. It can be used in the study of metabolism of mephenytoin and the activity of hepatic drug CYP2C19 in human