X-ray charge-coupled-device(CCD) camera working in single photon counting mode is a type of x-ray spectrometer with high-sensitivity and superior signal-to-noise performance. In this study, two single photon counting CCD cameras with the same mode(model: PI-LCX: 1300) are calibrated with quasi-monochromatic x-rays from radioactive sources and a conventional x-ray tube. The details of the CCD response to x-rays are analyzed by using a computer program of multi-pixel analyzing and event-distinguishing capability. The detection efficiency, energy resolution, fraction of multi-pixel events each as a function of x-ray energy, and consistence of two CCD cameras are obtained. The calibrated detection efficiency is consistent with the detection efficiency from Monte Carlo calculations with XOP program. When the multi-pixel event analysis is applied, the CCDs may be used to measure x-rays up to 60 ke V with good energy resolution(E /?E ≈ 100 at60 ke V). The difference in detection efficiency between two CCD cameras is small(5.6% at 5.89 ke V), but the difference in fraction of the single pixel event between them is much larger(25% at 8.04 ke V). The obtained small relative error of detection efficiency(2.4% at 5.89 ke V) makes the high accurate measurement of x-ray yield possible in the laser plasma interaction studies. Based on the discrete calibration results, the calculated detection efficiency with XOP can be used for the whole range of 5 ke V–30 ke V. X-ray charge-coupled-device (CCD) camera working in single photon counting mode is a type of x-ray spectrometer with high-sensitivity and superior signal-to-noise performance. In this study, two single photon counting CCD cameras with the same mode (model: PI-LCX: 1300) are calibrated with quasi-monochromatic x-rays from radioactive sources and a conventional x-ray tube. The details of the CCD response to x-rays are analyzed by using a computer program of multi-pixel analyzing and event-distinguishing capability. The detection efficiency, energy resolution, fraction of multi-pixel events each as a function of x-ray energy, and consistence of two CCD cameras are obtained. The calibrated detection efficiency is consistent with the detection efficiency from Monte Carlo calculations with XOP program. When the multi-pixel event analysis is applied, the CCDs may be used to measure x-rays up to 60 ke V with good energy resolution (E /? E ≈ 100 at 60 keV) The difference in detection efficiency The two small CCD cameras were small (5.6% at 5.89 keV), but the difference in fraction of the single pixel event between them is much larger (25% at 8.04 keV) at 5.89 ke V) makes the high accurate measurement of x-ray yield possible in the laser plasma interaction studies. Based on the discrete calibration results, the calculated detection efficiency with XOP can be used for the whole range of 5 ke V-30 ke V.