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基于不同类型膜的分离机理,构建了壳寡酸-无机盐混合体系的分离技术路线:首先用超滤膜除去壳寡酸混合体系中大部分无机盐和不具备生物活性的小分子壳寡酸;进一步用纳滤膜和透析膜除盐,制备分子量为1000~3500的壳寡酸产品。壳寡酸原始样品中无机盐成分与含量用离子色谱定量检测,结果显示,样品所含的无机盐阴离子为SO2-4、PO3-4和NO-3,其在单位质量样品中的含量分别为171.9、297.04和129mg/g。采用电导率仪检测壳寡酸混合溶液中无机阴离子的总含量,其精度经离子色谱验证,结果显示,当透过液电导率小于5μs/cm时,经透析分离制备的高纯壳寡酸标准品检测不到无机阴离子。首次分离出具有活性功能的壳寡酸标准品,为壳寡酸的工业生产提供了参照标准。
Based on the separation mechanism of different types of membranes, the separation technology of chitosan oligosaccharides-inorganic salts mixed system was established. Firstly, the most inorganic salts in the mixed system of chitosan oligosaccharides and the small molecule chitosan oligosaccharides ; Further use of nanofiltration and dialysis membrane desalination, preparation of 1000 ~ 3500 molecular weight of the shell oligosaccharides products. The results showed that the content of inorganic anions contained in the sample was SO2-4, PO3-4 and NO-3, and their contents in unit mass samples were 171.9, 297.04 and 129 mg / g. The total content of inorganic anions in the mixed solution of chitosan oligosaccharides was detected by conductivity meter and the accuracy was confirmed by ion chromatography. The results showed that the high purity chitosan oligoacid standard prepared by dialysis was used when the liquid conductivity was less than 5μs / cm Product detected no inorganic anions. For the first time, the active standard chitosan oligosaccharide was isolated, which provided a reference standard for the industrial production of chitosan oligosaccharides.