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目的:通过表达谱基因芯片研究蝎毒多肽提取物PESV对H22肝癌基因表达的影响,探讨其可能的药物作用机理。方法:建立H22肝癌皮下荷瘤模型,将60只昆明小鼠随机分为模型组、PESV组、Gemcitabine和TNP-40组,每组15只;无菌摘取皮下肿瘤组织,提取总RNA,通过反转录方法制备cDNA荧光探针,进行芯片杂交,然后对芯片进行图像扫描,最后用Imagene4.0软件包对荧光信号进行分析。结果:蝎毒多肽提取物PESV作用于荷瘤小鼠后,有127个基因表达下调,433个基因表达明显上调,其中35个基因与抗肿瘤血管生成药物TNP-470处理组下调基因一致,有40个与化疗药物Gemcitabine处理组下调基因一致。结论:蝎毒多肽提取物PESV可能同时具有细胞毒作用和抗血管生成作用双重作用,有望成为一种极具前景的肿瘤治疗药物。
OBJECTIVE: To study the effect of PESV, a scorpion venom polypeptide extract, on the gene expression of H22 hepatocellular carcinoma (H22) by expression gene microarrays and explore its possible mechanism of action. Methods: H22 hepatocarcinoma subcutaneous tumor model was established. Sixty Kunming mice were randomly divided into model group, PESV group, Gemcitabine and TNP-40 group, with 15 rats in each group. Subcutaneous tumor tissue was extracted aseptically and total RNA was extracted. Reverse transcription method cDNA fluorescent probe was prepared, the chip hybridization, and then the chip image scanning, and finally using the Imagene4.0 package on the fluorescence signal analysis. Results: PESV of scorpion venom peptide extract reduced the expression of 127 genes and increased the expression of 433 genes in tumor-bearing mice. Among them, 35 genes were down-regulated with the anti-tumor angiogenesis drug TNP-470, with 40 were consistent with the down-regulated genes of the chemotherapy drug Gemcitabine treatment group. CONCLUSION: PESV, a polypeptide extract from scorpion venom, may play dual roles of both cytotoxicity and anti-angiogenic effect, which is expected to become a promising oncology drug.